Coding

Part:BBa_K2012002:Design

Designed by: Pan Chu   Group: iGEM16_HZAU-China   (2016-09-04)


PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 14
    Illegal BamHI site found at 1172
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 577
    Illegal AgeI site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 87


Design Notes

codon optimized, and could express protein in Escheriachia coli.


Source

PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain, in its activated form.


References